Effects of Borax and Sleeve Gastrectomy on mRNA Expression of Antioxidant Genes in Substantia Nigra Tissue of Obese Rats

To evaluate the effects of borax and sleeve gastrectomy on mRNA expression of antioxidant genes in substantia nigra tissue of obese rats. Methods: Obese rats were fed with a high-fat diet containing 40% additional fat to the diet. Rats were allocated into four groups in random, which were normal rats (Group I) (n=14), obese rats subjected to SG (Group II) (n=14), obese rats subjected to borax (Group III) (n=14), and obese rats subjected to SG and borax (Group IV) (n=14).

Catalase, superoxide dismutase (SOD) and glutathione S-transferase (GST) gene expressions were determined by polymerase chain reaction, real-time polymerase chain reaction (RT-qPCR) and western blotting.

When normal rats (Group I), and obese rats subjected to SG (Group II) were compared, a decrease in expressions of catalase, SOD and GST genes was observed in Group II. When obese rats subjected to borax (Group III) were compared with Group I and Group II, a decrease in expressions of catalase, SOD and GST genes was observed in Group III.

PDF LINK