To evaluate the effects of borax and sleeve
gastrectomy on mRNA expression of antioxidant genes in substantia nigra tissue
of obese rats. Methods: Obese rats were fed with a high-fat diet containing 40%
additional fat to the diet. Rats were allocated into four groups in random,
which were normal rats (Group I) (n=14), obese rats subjected to SG (Group II)
(n=14), obese
rats subjected to borax (Group III) (n=14), and obese rats subjected to SG
and borax (Group IV) (n=14).
Catalase, superoxide dismutase (SOD) and
glutathione S-transferase (GST) gene expressions were determined by polymerase
chain reaction, real-time polymerase chain reaction (RT-qPCR) and western
blotting.
Results: When normal rats (Group I), and obese
rats subjected to SG (Group II) were compared, a decrease in expressions of
catalase, SOD and GST genes was observed in Group II. When obese rats subjected
to borax (Group III) were compared with Group I and Group II, a decrease in
expressions of catalase, SOD and GST genes was observed in Group III. This
phenomenon demonstrates that borax and SG both decrease expressions of catalase,
SOD and GST genes.
